HomeProductsForensic ScienceMicroreader™ 21 Direct ID System

Microreader™ 21 Direct ID System

Classical commercial kit which has been used for more than 10 years domestically and worldwide.
Allows direct amplification from these common sample types used in paternity testing without the need for extraction or purification, such as blood and buccal samples deposited on swab or paper substrates, as well as extracted DNA from various sample.
Superior discrimination ability, with cumulative non-father exclusion rate CPE>0.999999995.
Improved genotyping accuracy through superior primer design, expanded allelic ladder and virtual bins.
Greatly improves the discrimination for special and complex kinship relationship.

Product Details

The Microreader™ 21 Direct ID System is a 5-dye direct-amplification STR kit that amplifies 20 STR loci (including 13 CODIS loci) as well the highly discriminating Penta D and Penta E loci and a gender marker: Amelogenin formaximum concordance with existing kits and high confidence in paternity DNA test conclusions.

The Kit is subjected to rigorous quality control testing to offer consistent performance. We develop and manufacture our products in accordance with ISO 9001 and ISO 18385 quality system requirements.


Performance

Superior discrimination performance

It conforms to international standards and the forensic scientific DNA database construction specifications, has a high individual recognition rate, and its cumulative non-father exclusion rate is CPE>0.999999995, which is suitable for Paternity DNA test, DNA database construction, and other forensic analysis.


High quality reproductivity

This kit enables high-quality direct PCR amplification of reference samples with an improved first-pass success rate. You can expect: ① Well-resolved peaks above the background noise level ② Minimal occurrence of allelic drop-out, off-ladder allele calls, and off-scale data ③ Well-balanced profile within each dye color for maximized Intracolor Balance (ICB) values.


Highly Accurate
This kit is independently developed, selecting highly polymorphic genetic loci; through reasonable loci arrangement, enable the maximum length of the amplified product less than 410bp, and all amplified products are within the best detection range of the detection. Make sure the most accurate and reliable genetic information can be obtained.

Direct amplification & higher efficiency

Without the need for sample extraction, purification and quantification, the entire process from direct amplification of the samples to the PCR product can be completed in 90 minutes, which improves the work efficiency and reduced testing costs, which are very suitable for the processing of large batches of samples.


Anti-pollution design

The kit has an anti-pollution design. When performing STR typing it can remove other PCR products that may be mixed in the amplification tube, avoid personnel contamination or non-specific amplification, and effectively reduce the impact of laboratory aerosol contamination. Increase the success rate of the experiment.


Kit related published paper

[1] Wu, Heming, et al. "Invasive molecular prenatal diagnosis of alpha and beta thalassemia among Hakka pregnant women." Medicine 97.52 (2018). (DOI: 10.1097/MD.0000000000013557) [2] Yang, Yaran, et al. "Haplotypic polymorphisms and mutation rate estimates of 22 Y-chromosome STRs in the Northern Chinese Han father–son pairs." Scientific reports 8.1 (2018): 1-6. (DOI: 10.1038/s41598-018-25362-3).


Kit dyes and corresponding markers

D19S433D5S818D21S11D18S51D6S1043
AmelD3S1358D13S317D7S820D16S539CSF1POPenta D
D2S441vWAD8S1179TPOXPenta E
TH01D12S391D2S1338FGA

Kit Specifications

For Use with (Application) sequencing Unit size 100/200 reactions
Label or Dye 5 Dye Product type PCR amplification Kit

Detection method

Capillary Electrophoresis

Target Loci

D19S433, D5S818, D21S11, D18S51, D6S1043, AMEL, D3S1358, D13S317, D7S820, D16S539, CSF1PO, PENTA D, D2S441, VWA, D8S1179, TPOX, PENTA E, TH01, D12S391, D2S1338, FGA

Shipping condition & Storage

Shipping condition: Dry ice
Storage: Store at –5 to –30°C upon receipt and at 2–8°C after initial use.

Documents & Downloads

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