The Microreader™ 21 Direct ID System is a 5-dye direct-amplification STR kit that amplifies 20 STR loci (including 13 CODIS loci) as well the highly discriminating Penta D and Penta E loci and a gender marker: Amelogenin formaximum concordance with existing kits and high confidence in paternity DNA test conclusions.
The Kit is subjected to rigorous quality control testing to offer consistent performance. We develop and manufacture our products in accordance with ISO 9001 and ISO 18385 quality system requirements.
It conforms to international standards and the forensic scientific DNA database construction specifications, has a high individual recognition rate, and its cumulative non-father exclusion rate is CPE>0.999999995, which is suitable for Paternity DNA test, DNA database construction, and other forensic analysis.
This kit enables high-quality direct PCR amplification of reference samples with an improved first-pass success rate. You can expect: ① Well-resolved peaks above the background noise level ② Minimal occurrence of allelic drop-out, off-ladder allele calls, and off-scale data ③ Well-balanced profile within each dye color for maximized Intracolor Balance (ICB) values.
Without the need for sample extraction, purification and quantification, the entire process from direct amplification of the samples to the PCR product can be completed in 90 minutes, which improves the work efficiency and reduced testing costs, which are very suitable for the processing of large batches of samples.
The kit has an anti-pollution design. When performing STR typing it can remove other PCR products that may be mixed in the amplification tube, avoid personnel contamination or non-specific amplification, and effectively reduce the impact of laboratory aerosol contamination. Increase the success rate of the experiment.
[1] Wu, Heming, et al. "Invasive molecular prenatal diagnosis of alpha and beta thalassemia among Hakka pregnant women." Medicine 97.52 (2018). (DOI: 10.1097/MD.0000000000013557) [2] Yang, Yaran, et al. "Haplotypic polymorphisms and mutation rate estimates of 22 Y-chromosome STRs in the Northern Chinese Han father–son pairs." Scientific reports 8.1 (2018): 1-6. (DOI: 10.1038/s41598-018-25362-3).
For Use with (Application) | sequencing | Unit size | 100/200 reactions |
---|---|---|---|
Label or Dye | 5 Dye | Product type | PCR amplification Kit |
Detection method |
Capillary Electrophoresis |
Target Loci |
D19S433, D5S818, D21S11, D18S51, D6S1043, AMEL, D3S1358, D13S317, D7S820, D16S539, CSF1PO, PENTA D, D2S441, VWA, D8S1179, TPOX, PENTA E, TH01, D12S391, D2S1338, FGA |