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Microreader™ 28A ID System

Cover all CODIS and ESS markers, for maximum global database compatibility.
Highly Sensitive: complete STR profiles could be obtained when the DNA template content was as low as 0.0625ng.
The inclusion of 10 mini-STRs maximizes results from degraded samples.
Improved performance with degraded samples and PCR inhibitors.

Product Details

Microreader™ 28A ID System with advanced 6-dye fluorescent, this kit amplifies 24 autosome STR loci, 1 Y chromosome STR loci, 2 Y-indel loci and Amlogenin. This kit is compatible with the commonly used commercial autosome casework kits. For casework Y-chromosome kit, please see "Microreader™ Y Prime ID System" in same catalogue, or "Microreader™ Y Prime Plus ID System" in catalogue "Forensic DNA database solutions".

The Kit is subjected to rigorous quality control testing to offer consistent performance. We develop and manufacture our products in accordance with ISO 9001 and ISO 18385 quality system requirements.


Performance

Highly Sensitive
PCR components and conditions are well optimized, which greatly improves sensitivity of the kit. Full profile could still be obtained with DNA input as low as 0.0625 ng.10 mini-STR (<220bp) could be detected by specifically designed STR loci arrangement, which are very powerful when dealing with degraded samples.

Stronger ability of inhibition resistance
On the basis of high sensitivity, the improved buffer formula obtained higher anti-PCR inhibitor capability and higher amplification efficiency for samples containing humic acid, heme, tannic acid and other DNA inhibitors, ensuring stable and efficient amplification and obtainment of more reliable genotyping data.

Elaborately design of primers
In the R&D process, the primer design process was greatly improved and combined with the new fluorescence. High quality primers can effectively improve the balance of different dyes, reduce the generation of dye peaks, and help analysts to analyze data more easily and quickly, and improve work efficiency.

Kit dyes and corresponding markers

TH01D5S818D21S11D18S51D6S1043
Y-indelAmelD3S1358D13S317D7S820D16S539CSF1POPenta D
D2S441VWAD8S1179TPOXPenta EY-indel
D19S433D22S1045D2S1338FGA
DYS391D1S1656D12S391D10S1248SE33

Kit Specifications

For Use with (Application) sequencing Unit size 100/200 reactions
Label or Dye 6 Dye Product type PCR amplification Kit
Detection method Capillary Electrophoresis Target Loci TH01, D5S818, D21S11, D18S51, D6S1043, Y-indel, Amel, D3S1358, D13S317, D7S820, D16S539, CSF1PO, Penta D, D2S441, VWA, D8S1179, TPOX, Penta E, Y-indel, D19S433, D22S1045, D2S1338, FGA, DYS391, D1S1656, D12S391, D10S1248, SE33
Required ESS markers Covered Required CODIS markers Covered

Shipping condition & Storage

Shipping condition: Dry ice
Storage: Store at –5 to –30°C upon receipt and at 2–8°C after initial use.

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