Short tandem repeat (STR) genotyping detection combined with multiplex fluorescent PCR technology is used to identify maternal cell contamination in samples for prenatal diagnosis of genetic diseases and investigation of causes of miscarriage. It has strong specificity and sensitivity, and the results are accurate It is reliable and can better solve the problem of individual identification in mixed samples.

Maternal cell contamination identification, detection of 10% maternal cell contamination

• In 2008, the British Clinical Molecular Genetics Society (CMGS) formulated the MCC specification for prenatal diagnostic specimen molecular testing and suggested that all prenatal diagnosis results for single gene diseases must include MCC differential tests.
  

• In 2013, the American College of Medical Genetics and Genomics (ACMG) recommended that all prenatal samples should be analyzed for MCC.
  

• In 2019, the "Expert Consensus on the Application of Low-depth Whole-Genome Sequencing Technology in Prenatal Diagnosis" clearly suggested: Combine CNV-seq technology with fluorescent quantitative PCR (QF-PCR) or STR detection, and STR detection can be used to detect Prenatal samples were used for MCC determination.
  

• In 2020, the "Consensus on Data Analysis, Interpretation and Reporting Standardization of Copy Number Variation and Homozygous Regions in Prenatal Genetic Diagnosis" clearly recommends that all prenatal diagnosis specimens undergoing CMA/CNV-seq should be tested for STR or single nucleotide Polymorphism typing and other detection methods to exclude maternal DNA contamination.
  

• Avoid wrong diagnostic results of prenatal diagnosis due to maternal cell contamination, resulting in adverse birth outcomes and medical disputes;
  

• Avoid miscarriage cause investigation errors, causing patients to receive unnecessary other examinations and wrong treatment plans.
  

Maternal Cell Contamination (MCC) of prenatal diagnosis and abortion tissue samples identification

• Tissue samples (amniotic fluid, cord blood, chorion, abortion tissue), blood and extracted genomic DNA samples
  

Amplify 21 STR genetic loci in a single tube to improve detection accuracy

Highly polymorphic, distantly located on chromosomes, no linkage affect

Hundreds of thousands of sample tested, great repeatability

Single tube amplification, complete PCR within 1.5h, and a complete STR profile can be obtained as low as 1ng of DNA

PCR instrument + Genetic analyzer

PCR instrument: Life Technologies Holdings Pte Ltd: Veriti, Veriti Dx, 9700

Genetic analyzer: Life Technologies Holdings Pte Ltd: 3500 Dx, 3500 xL Dx; Seqstudio.

Multiplex fluorescent PCR-capillary electrophoresis

[1] Allen S, Mountford R , Butler A , et al. Practice Guidelines for the Testing for Maternal Cell Contamination (MCC) in Prenatal Samples for Molecular Studies. Guidelines ratified by the UK Clinical Molecular Genetics Society (2008).

[2] Sarah, T., et al. "ACMG Standards and Guidelines for constitutional cytogenomic microarray analysis, including postnatal and prenatal applications: revision 2013. " Genetics in medicine : official journal of the American College of Medical Genetics (2013).

[3] Expert consensus on the application of low-depth whole-genome sequencing technology in prenatal diagnosis [J]. Chinese Journal of Medical Genetics, 2019, 36(4):293-296.

[4] Consensus on data analysis, interpretation and reporting standardization of prenatal genetic diagnosis of copy number variation and homozygous regions [J]. Chinese Journal of Medical Genetics, 2020, 37(07):701-708.

※  This product is only for scientific research use, and this information is only for reference by relevant medical professionals. Please refer to the instruction manual for details of contraindications or precautions.

[ENG]Maternal_blood_contamination.pdf:Reference:[ENG]Maternal_blood_contamination.pdf