Prevention of birth defects, unhealthy pregnancy, pregnancy-induced hypertension etc.
Prevention of birth defects, unhealthy pregnancy, pregnancy-induced hypertension etc.
Folic acid plays an important role in the growth and development of the human body. The human body cannot synthesize it by itself and must be ingested from the outside world. Insufficient folic acid supplementation or poor utilization ability will cause folic acid deficiency and lead to hyperhomocysteinemia. The lack of folic acid in pregnant women can lead to birth defects, and pregnant women themselves will also have many adverse symptoms. However, excessive supplementation of folic acid can also cause various side effects, and both excess and deficiency may cause problems.
No need to extract DNA, save time and effort, reduce pollution.
Contains 2 key genes affecting folic acid metabolism, a total of 3 genetic loci.
Professional software assisted analysis and interpretation of results, fast and easy.
Single-tube amplification, the whole process only takes 3 hours, suitable for automatic and batch detection.
Effectively avoid PCR product contamination.
Tests and studies have shown that different populations have different abilities to utilize folic acid, so the required supplement doses are also different. 5,10-methylenetetrahydrofolate reductase (MTHFR) and methionine synthase reductase (MTRR) are key enzymes that affect folic acid metabolism, and their gene polymorphisms are responsible for the differences in folic acid utilization among individuals main reason. When the MTHFR or MTRR gene is mutated, the activity of its enzyme is reduced, which leads to the disorder of folic acid metabolism, and it is necessary to supplement more folic acid than ordinary people to maintain the normal demand of the human body.
In 2008, the Maternal and Child Health Center of the Chinese Center for Disease Control and Prevention listed folic acid utilization ability genetic testing and risk assessment as a clinical application guideline, suggesting that mothers-to-be should conduct folic acid utilization ability genetic testing before supplementing folic acid, and choose a personalized supplement plan based on risk classification. In addition, the detection of MTHFR and MTRR gene polymorphisms also has certain guiding significance for patients with hyperhomocysteinemia to supplement folic acid and reduce their risk of stroke.
The c.677 and c.1298 sites of the MTHFR gene and the c.66 site of the MTRR gene that affect folic acid metabolism are amplified by multiplex fluorescent PCR, combined with capillary electrophoresis detection, and professional software is provided for analysis and interpretation of the results.
1 Sample collection
2 Multiplex fluorescent PCR amplification
3 Capillary electrophoresis detection
4 Result analysis
PCR machine: Life Technologies Holdings Pte Ltd：Veriti, Veriti Dx,9700
Genetic Analyzer: Life Technologies Holdings Pte Ltd: 3500, Seqstudio
[1] Luo Li, et al. The relationship between folate metabolism gene polymorphism and birth defects. International Journal of Obstetrics and Gynecology. 2015(4):421-424.
[2] "Compilation of Clinical Genetic Testing Items for Maternal and Child Health Care" Volume 6 Clinical Application Guidelines No. 21 "Folic Acid Utilization Ability"
[3] Deng Yingfang, et al. Analysis of folic acid deficiency and MTHFR, MTRR gene polymorphisms in hyperhomocysteine (Hcy) patients. Chinese Journal of Molecular Cardiology. 2015(5):1463-1465.
※This product is only for scientific research use, and this information is only for reference by relevant medical professionals. Please refer to the instruction manual for details of contraindications or precautions.
[ENG]MTHFR_MTRR.pdf:Reference:[ENG]MTHFR_MTRR.pdf
