HomeProductsForensic ScienceMicroreader™ 23sp Direct ID System

Microreader™ 23sp Direct ID System

Allows direct amplification from these common sample types used in paternity testing without the need for extraction or purification, such as blood and buccal samples deposited on swab or paper substrates, as well as extracted DNA from various sample.
Superior discrimination ability, with cumulative non-father exclusion rate CPE>0.999 999 999.

Product Details

Microreader™ 23sp Direct ID System is a forensic grade product with 5-dye fluorescent technology and a co-amplification method to detect 23 loci including 21 non-CODIS loci, 1 CODIS loci, and Amelogenin. It is specifically designed for supplementary STR testing for paternity and kinship testing in complex relationship and to obtain a huge amount of information.

The Kit is subjected to rigorous quality control testing to offer consistent performance. We develop and manufacture our products in accordance with ISO 9001 and ISO 18385 quality system requirements.


Performance

Superior Discrimination Performance
As an addition to regular paternity DNA test, Microreader™ 23sp Direct ID System greatly improves probability of discrimination power (DP) and excluding probability of paternity (PE) due to the choice of non-linked loci. The cumulative non-father exclusion rate CPE>0.999 999 999.

Highly Informative
It can be used with Microreader™ 21 Direct ID System to detect up to 39 autosomal STR loci. Enable the comprehensive detection of supplementary STR loci.

Direct Amplification & Higher Efficiency
Without the need for sample extraction, purification and quantification, the entire process from direct amplification of the samples to the PCR product can be completed in 90 minutes, which improves the work efficiency and reduced testing costs, which are very suitable for the processing of large batches of samples.

High Compatibility
As an autosomal complementary paternity test Kit, it can be used together with the mainstream kits in the market, such as ABI: Globalfiler, Promega: VersaPlex 27PY, etc, from the angle of phylogenetic identification, increase the accuracy of screening.

Kit Related Published Paper
[1] Wen, Dan, et al. "Genetic polymorphisms of new 22 autosomal STR loci in the Mongolian ethnic group." International journal of legal medicine 133.5 (2019): 1405-1407. (DOI: 10.1007/s00414-019-02111-3) [2] Liu, Qiu-Ling, et al. "Genetic polymorphism of 22 autosomal STR markers in a Han population of Southern China." Forensic Science International: Genetics 24 (2016): e14-e16. (DOI: 10.1016/j.fsigen.2016.06.017)

Kit dyes and corresponding markers

D6S477D18S535D19S253D15S659D11S2368D20S470
AmelD1S1656D22-GATA198B05D16S539D7S3048D8S1132
D4S2366D21S1270D13S325D9S925D3S3045D14S608
D10S1435D12S391D2S1338D17S1290D5S2500

Kit Specifications

For Use with (Application) sequencing Unit size 100/200 reactions
Label or Dye 5 Dye Product type PCR amplification Kit
Detection method Capillary Electrophoresis Target Loci

D6S477, D18S535, D19S253, D15S659, D11S2368, D20S470, AMEL, D1S1656, D22-GATA198B05, D16S539, D7S3048, D8S1132, D4S2366, D21S1270, D13S325, D9S925, D3S3045, D14S608, D10S1435, D12S391, D2S1338, D17S1290, D5S2500

Shipping condition & Storage

Shipping condition: Dry ice
Storage: Store at –5 to –30°C upon receipt and at 2–8°C after initial use.

Documents & Downloads

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